Agarose powder

Agarose is a standard melting temperature, multi-purpose agarose that is ideal for routine separation analysis.

Agarose resolves DNA and RNA fragments from 500–23,000 bp, and has no detectable DNase or RNase activity. It can also be used for:

6X DNA Loading Dye

The 6X gel loading buffer is composed of 0.03% Bromophenol Blue, 0.03% Xylene cyanol FF, 60 mM EDTA, pH 7.6 and 60% glycerol calcium or magnesium in molecular biology grade water. The EDTA is added as a chelating agent to terminate enzymatic reactions requiring magnesium

Adding 1 volume of DNA Gel Loading Buffer to 9 or 5 volumes of sample increases the density of the sample and gives it blue color, thus facilitating loading. In addition, the two dyes migrate in the same direction as nucleic acids, serving as rough indicators of the electrophoretic progress.

RNA Loading Dye

2X RNA Loading Dye is recommended for preparation of RNA samples for electrophoresis on agarose or polyacrylamide gels.

It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide.

In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA.2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilizes RNA.

RNA Stabilization solution

Obtaining high quality, intact RNA is the first and often the most critical step in performing gene expression analysis.

Typically, in order to isolate high quality RNA, the tissue has to be processed immediately after harvest. RNA Solution makes it possible for researchers to postpone RNA isolation for days, weeks, or even months after tissue collection without sacrificing RNA integrity.

All we need to do is to add 10 times volume of RNA Solution into the tube containing the freshly collected tissue (1 ml RNA Solution to 100 mg tissue) and store the tube at –20℃ until use. In addition for RNA stabilization, RNA Solution can be easily integrated into a modified single-step RNA isolation method.