PCR Taq Mixture

PCR Master Mix is a premixed, ready to use solution containing Taq DNA polymerase, dNTPs, and MgCl2 and reaction buffer at optimal temperature for efficient amplification of DNA templates by PCR. PCR Master contains blue dye that allows monitoring of progress during electrophoresis.

Taq DNA Polymerase

Taq DNA polymerase is a thermostable enzyme of approximately 94 kDa purified from the expression product of E.coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT1.

This unmodified enzyme replicates DNA at 74 °C and exhibits a half-life of 40 minutes at 95 °C . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5’      3’ direction in the presence of magnesium and also possesses a 5’    3’ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

dNTPs

A ready to use molecular grade dNTP mix containing dATP, dCTP, dGTP and dTTP (each 10 mM). The mix is designed to save hands on time for researchers and minimize the possibility of contamination.