Umbrella life science’s advanced technology has led to the invention of high-quality RT-PCR reagents, including M-MLV Reverse Transcriptase ,cDNA Synthesis Kit , RT-PCR Kit, 2X Sybr green Mix and more. They simply provide you with instant access to high throughput PCR automation, offering cost-effective solution and satisfactory results.

Minus M-MLV Reverse Transcriptase

MMLV Reverse Transcriptase (MMLV-RT), encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the cDNA first strand from a single-stranded RNAtemplate to which a primer has been hybridized. MMLV-RT will also extend primers hybridized tosingle-stranded DNA.

1st Strand

1st Strand cDNA Synthesis Kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TRUEScript Hˉ RTase /RI Mix and 5X RT Reaction Mix(dNTP included ). Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.cDNA up to 12 kb.

One-Step RT-PCR Kit

One-Step RT-PCR Kitis designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 25 or 100 amplification reactions of 50 μl each.

2× SYBR Green qPCR Mix

2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes.

SYBR Green qRT-PCR Kit

The SYBR Green RT-PCR Kit provides highly specific quantification of RNA targets by real-time one-step RT-PCR using SYBR Green I detection. The combination of a hot start and a unique qRT-PCR buffer system ensures highly specific and sensitive real-time quantification of RNA targets. The dNTP mix includes dUTP, allowing optional treatment with UNG. The SYBR Green RT-PCR Kit is also supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts. For convenience, the master mix can be stored at 2–8°C.